Arrange the following steps of somatic hybridisation in a correct sequence. A. Digestion of cell walls. B. Isolation of naked protoplasts. C. Fusion of protoplasts to get hybrid protoplast. D. Isolation of single cells from two different varieites of plants. E. Growing of hybrid protoplast to form a new plant. Choose the correct answer from the options given below:

Core Logic

Somatic hybridization involves fusing protoplasts of two different plant varieties to create a hybrid. The logical steps are:

• Isolate single cells from the two desired plant varieties (D).
• Digest their cell walls using enzymes like pectinase and cellulase (A).
• Isolate the resulting naked protoplasts (B).
• Fuse these protoplasts (using chemicals like PEG or electrofusion) to obtain a hybrid protoplast (C).
• Grow/culture the hybrid protoplast to form a new plant (E).

Step 1: Final Conclusion

The correct sequence is D → A → B → C → E.

Pattern Recognition

You must isolate cells (D) before you digest walls (A). The last step is always growing the new plant (E). D,A,B,C,E fits perfectly.

Chapter Mix

Class 12 Biology: Strategies for Enhancement in Food Production