Q127 (2024)
Arrange the following steps of somatic hybridisation in a correct sequence.
A. Digestion of cell walls.
B. Isolation of naked protoplasts.
C. Fusion of protoplasts to get hybrid protoplast.
D. Isolation of single cells from two different varieites of plants.
E. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:
- E, A, B, C, D
- D, A, B, C, E
- E, B, A, D, C
- D, B, A, E, C
### Related Formula
Somatic Hybridisation Sequence:
Isolate Cells $\rightarrow$ Cell Wall Digestion $\rightarrow$ Naked Protoplasts $\rightarrow$ Protoplast Fusion $\rightarrow$ Regeneration of Plant.
### Core Logic
Steps in order:
1. Isolation of single cells from plant varieties (D)
2. Digestion of cell walls using pectinase/cellulase (A)
3. Isolation of naked protoplasts (B)
4. Fusion of protoplasts via PEG/electrofusion (C)
5. Regeneration/growing of hybrid protoplast into plant (E)
### Step 1: Conclusion
Sequence is D $\rightarrow$ A $\rightarrow$ B $\rightarrow$ C $\rightarrow$ E. Correct option is (2).
### Pattern Recognition
Initial step is cell isolation (D); final step is growing plant (E). Order: D, A, B, C, E.
### Evaluation Rubric / Model Answer
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### Chapter Mix
Class 12 Biology: Strategies for Enhancement in Food Production
