Arrange the following steps of somatic hybridisation in a correct sequence. A. Digestion of cell walls. B. Isolation of naked protoplasts. C. Fusion of protoplasts to get hybrid protoplast. D. Isolation of single cells from two different varieites of plants. E. Growing of hybrid protoplast to form a new plant. Choose the correct answer from the options given below:

Related Formula

Somatic Hybridisation Sequence: Isolate Cells arrow$\rightarrow$ Cell Wall Digestion arrow$\rightarrow$ Naked Protoplasts arrow$\rightarrow$ Protoplast Fusion arrow$\rightarrow$ Regeneration of Plant.

Core Logic

Steps in order:

• Isolation of single cells from plant varieties (D)
• Digestion of cell walls using pectinase/cellulase (A)
• Isolation of naked protoplasts (B)
• Fusion of protoplasts via PEG/electrofusion (C)
• Regeneration/growing of hybrid protoplast into plant (E)

Step 1: Conclusion

Sequence is D arrow$\rightarrow$ A arrow$\rightarrow$ B arrow$\rightarrow$ C arrow$\rightarrow$ E. Correct option is (2).

Pattern Recognition

Initial step is cell isolation (D); final step is growing plant (E). Order: D, A, B, C, E.

Chapter Mix

Class 12 Biology: Strategies for Enhancement in Food Production