Solution
Related Formula
DNA Fingerprinting Workflow: Isolation & Digestion arrow Gel Electrophoresis arrow Southern Blotting (Transfer) arrow Hybridization with Probe arrow Autoradiography.
Core Logic
Sequence of steps:
- Isolation and digestion of DNA by restriction enzymes (A)
- Separation of fragments by gel electrophoresis (E)
- Transferring (blotting) to synthetic membrane (C)
- Hybridisation using labelled VNTR probe (B)
- Detection by autoradiography (D)
Step 1: Conclusion
The correct order is A arrow E arrow C arrow B arrow D. Correct option is (1).
Pattern Recognition
Blotting (C) must happen BEFORE hybridization (B), and autoradiography (D) is the final detection step. Order: A, E, C, B, D.
Chapter Mix
Class 12 Biology: Molecular Basis of Inheritance